In February, BIOCOMES partner Agro Plantarum has collected soil for greenhouse trials. The soil will be used to test the antagonistic effects of two bacterial strains on Verticillium wilt disease on brassicas.
Agro Plantarum collected soil in the southern part of Sweden close to the village Billeberga. The soil was collected from a ‘healthy’ field and from a field that was naturally infested with Verticillium wilt. Verticillium longisporum is a pathogen that is often responsible for wilting diseases in Brassicaceae. The oilseed rape on the infested field showed severe symptoms of Verticillium wilt in 2013. We will use the infested soil to get these symptoms also in our greenhouse trial.
Photo 1: Agro Plantarum collects healthy soil from a field in Sweden.
At the same time a researcher of Wageningen UR, treated cauliflower seeds and seeds from three oil seeds rape cultivars with bacterial biocontrol strains provided by TU Graz.
Photo 2: Drying seeds after biopriming with bacterial strains at Wageningen UR.
At the end of February, the seeds were planted in pots in the greenhouse in Sweden with healthy soil and naturally and artificially infested soil with Verticillium. In this way we will test the antagonistic effects of the biocontrol bacteria on Verticillium as well as on host plants. Later we will perform field experiments.
Photo 3: Collected soil in the greenhouse in Sweden.
With these experiments the BIOCOMES partners work on the development of a seed treatment for oilseed rape and Brassica vegetables to control Verticillium wilt based on the biocontrol bacteria Serratia plymuthica and Paenibacillus polymyxa. Besides the activities in the greenhouse in 2015, scientists of TU Graz work on the optimisation of the seed treatment protocol.
Read more about our work on Brassica diseases
More info about soil-borne Verticillium wilt
BCA development against Brassica diseases
Soil-borne Verticillium wilt diseases in oil seed rape and vegetables