BCA 2 against Brown rot
- To characterize both biological control agents in order to meet Directive 2001/36 EEC requirements and to develop products suitable to be registered and commercialized
- To optimize the production processes of biological control agents to obtain commercially feasible scaling-up and downstream processing protocols
- To develop formulation strategies for each biological control agent to prolong shelf life and improve efficacy against brown rot in stone fruit under practical conditions
- To develop and validate control strategies based on the biological control agents, to determine doses, cost-effectiveness and application times adapted to different European stone fruit producing regions.
Bacillus subtilis CPA-8
Isolate CPA-8 was isolated by the IRTA research group from nectarine surface. It showed good efficacy against brown rot on stone fruit and other fungal fruit pathogens. The strain produces endospores that allow its survival under stress conditions, which is an important feature for a biocontrol agent.
Studies on the mode of action of CPA-8 showed that the antifungal substance fengycine (typical from this species) plays an important role in the biocontrol potential of the strain.
A laboratory scale liquid production system achieves good production in a cheap medium based on by-products from the food industry. A spray-drying system has been tested to formulate the biocontrol product with some promising results, but other drying systems still need to be tested.
Both biocontrol agents, Penicillium frequentans 909 and Bacillus subtilis CPA-8, have shown good potential for development as commercial products against the brown rot of stone fruit.